Journal: PLoS ONE

Article Title: Transdifferentiation of Adipose-Derived Stem Cells into Keratinocyte-Like Cells: Engineering a Stratified Epidermis

doi: 10.1371/journal.pone.0080587

Figure Lengend Snippet: List of sense and anti-sense primers used for RT-PCR.

Article Snippet: For immunofluorescence microscopy, staining was performed using primary rabbit anti-human cytokeratin-14 (Covance Inc., Princeton, NJ); primary rabbit anti-human Stratifin antibody (Enzo Biomol, Framingdale, NY) and primary mouse anti-human involucrin (Abcam, Cambridge, MA) at 1∶1000 dilution and incubated overnight at 4°C.

Techniques:

Journal: PLoS ONE

Article Title: Transdifferentiation of Adipose-Derived Stem Cells into Keratinocyte-Like Cells: Engineering a Stratified Epidermis

doi: 10.1371/journal.pone.0080587

Figure Lengend Snippet: We evaluated ASC transdifferentiation into KLC by the expression of several keratinocyte markers such as, keratins, involucrin, stratifin and filaggrin. (a) mRNA expression of cytokeratin-5, cytokeratin-14, stratifin, involucrin and filaggrin in ASC, keratinocytes (K), fibroblasts (F), and KLC. Keratinocyte and fibroblasts were used as positive and negative control, respectively. The panel shows the absence with the exception of filaggrin of all other keratinocytes markers in ASC and fibroblasts, and the positive expression of these markers in KLC after ASC-transdifferentation, (Cytokeratin-5, 0.26±0.03 vs. 0.0008±0.0001; cytokeratin-14, 0.34±0.07 vs 0.001±0.000089; stratifin, 0.42±0.19 vs 0.000055±0.0000056 and involucrin, 1.3±0.9 vs 0.0008±0.0005; respectively). In the case of filaggrin, KLC showed almost a 3 fold up-regulation when compared to that of ASC (0.85±0.14 vs 0.29±0.01; respectively) **p<0.01; n = 3 independent experiments. Keratinocytes were normalized to 1 since these cells possess these markers. Statistical analysis was obtained comparing KLC to ASC. (b) Western blot analysis confirmed the mRNA findings. KLC showed the presence of cytokeratin-5, involucrin, filaggrin and stratifin proteins comparable to those from keratinocyte lysates, however no bands were detected in ASC or fibroblast lysates. (Arrows indicate Cytokeratin-5 band, according to its molecular weight. Upper bands may correspond to unspecific binding at a higher molecular weight not corresponding with cytokeratin-5 molecular weight) (Image representative n = 3 independent experiments).

Article Snippet: For immunofluorescence microscopy, staining was performed using primary rabbit anti-human cytokeratin-14 (Covance Inc., Princeton, NJ); primary rabbit anti-human Stratifin antibody (Enzo Biomol, Framingdale, NY) and primary mouse anti-human involucrin (Abcam, Cambridge, MA) at 1∶1000 dilution and incubated overnight at 4°C.

Techniques: Expressing, Negative Control, Western Blot, Molecular Weight, Binding Assay

Journal: PLoS ONE

Article Title: Transdifferentiation of Adipose-Derived Stem Cells into Keratinocyte-Like Cells: Engineering a Stratified Epidermis

doi: 10.1371/journal.pone.0080587

Figure Lengend Snippet: ASC were seeded in chamber slides and cultured in the presence of KCM. Cells were fixed and stained at different time points (Days 0, 7, 14, 28 and 42) for evaluation. (a) ASC were immunostained with anti-human cytokeratin-14. DAPI was used as counterstaining. Images demonstrate detection of cytokeratin-14 as early as day 7 post-treatment. Keratinocytes and ASC were used as positive and negative controls, respectively. (Image representative n = 3 independent experiments) (b) ASC were immunostained with anti-human Involucrin and anti-human Stratifin. DAPI was used as counterstaining. Expression of stratifin was detected starting at day 14. Expression of involucrin was observed starting at day 28. Keratinocytes and ASC were used as positive and negative controls, respectively. (Image representative n = 3 independent experiments). Quantification of transdifferentiated ASC into KLC. ASC cells were seeded on chamber slides and cultured in the presence of KCM. At different time points (Days 0, 7, 14, 28, and 42) cells were fixed and stained using anti-human cytokeratin-14, anti-human Stratifin and anti-human Involucrin. Slides were scanned using TissueFAXS system and quantified using TissueQuest software. Average of positive stained cells were divided by the average of total number of cells (nuclei counts) and multiplied by 100, to obtain the percentage. (*n = 3 slides per time point).

Article Snippet: For immunofluorescence microscopy, staining was performed using primary rabbit anti-human cytokeratin-14 (Covance Inc., Princeton, NJ); primary rabbit anti-human Stratifin antibody (Enzo Biomol, Framingdale, NY) and primary mouse anti-human involucrin (Abcam, Cambridge, MA) at 1∶1000 dilution and incubated overnight at 4°C.

Techniques: Cell Culture, Staining, Expressing, Software

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 expression is increased in renal clear cell carcinoma tissue samples and cells and correlates with poor prognosis. ( A – C ) The public database shows elevated expression of PDLIM7 in ccRCC. ( D ) Immunohistochemical staining shows elevated expression of PDLIM7 in ccRCC. ( E ) PDLIM7 expression in ccRCC cells was significantly higher than that in normal cells HK2. Scale bar:10 µm and 5 µm ( F , G ) Effect of PDLIM7 on OS and PFS in ccRCC patients.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: PDLIM7 promotes cell proliferation, migration, and invasion in ccRCC. ( A ) Validation of western blot assay for transfection with sh-PDLIM7 in 769-p and Caki-1 cells. ( B ) Cloning assay for ccRCC cell viability. ( C ) Detection of cellular wound healing capacity by scratch assay. Scale bar:500 µm ( D ) Transwell assay for cell migration and invasion. Scale bar:10 µm ( E ) Western blot for EMT-related protein expression.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Migration, Biomarker Discovery, Western Blot, Transfection, Cloning, Wound Healing Assay, Transwell Assay, Expressing

Journal: Scientific Reports

Article Title: The effect of PDLIM7 on cell proliferation, migration, and drug sensitivity in clear cell renal cell carcinoma

doi: 10.1038/s41598-025-11495-9

Figure Lengend Snippet: KEGG/GO functional enrichment and drug sensitivity. ( A ) Functional enrichment of PDLIM7 and related genes KEGG/GO. ( B ) PDLIM7 and drug correlation.

Article Snippet: PDLIM7 primary antibody(1:100,finetest,China), a DAB-enhanced chromogenic solution, and a rabbit two-step assay kit were obtained from Beijing Zhongsui Jinqiao Company.

Techniques: Functional Assay

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Chondrocyte gene network of CSGalNAcT-1 was classified as glycan biosynthesis and metabolism and functional pathway

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Functional Assay

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 mRNA expression in osteoarthritis (OA) cartilage, Kashin-Beck disease (KBD) cartilage and normal cartilage. CSGalNAcT-1 and Hapln-1 mRNA levels reach statistical significance. * Denotes significant difference at P < 0.05

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: Comparison of CSGalNAcT-1 expression in the cartilage from osteoarthritis (OA), Kashin-Beck disease (KBD) and normal groups. a , b , c Denote the upper of normal, OA and KBD cartilage, respectively. d , e , f Denote the middle of normal, OA and KBD cartilage, respectively. g , h , i Denote the deep of normal, OA and KBD cartilage, respectively

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: International Orthopaedics

Article Title: Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin–Beck disease and primary osteoarthritis

doi: 10.1007/s00264-013-1937-y

Figure Lengend Snippet: CSGalNAcT-1 and Hapln-1 protein level expression reduced in osteoarthritis (OA) and Kashin-Beck disease (KBD) cartilage compared with normal controls. Wnt 3a, β-catenin and Runx-2 level expression increased in OA and KBD cartilage compared with normal controls. A KBD free-Se group. B 0.25 μg/ml Se + KBD group. C 0.10 μg/ml Se + KBD group. D 0.05 μg/ml Se + KBD group

Article Snippet: For the primary antibody, polyclonal rabbit anti-human primary antibodies against CSGalNAcT-1 (1:50; ABGENT, San Diego, CA 92121, USA) and Hapln-1 (1:50; Abcam, UK) were used at 4 ° C overnight.

Techniques: Expressing

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Main immunohistochemical findings in the study groups

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Additional immunohistochemical findings (intensity score in alveolar walls and bronchiolar epithelium)

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining